| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093171 |
| Chromosome: | chromosome 15 |
| Location: | 1708007 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g643700 | RLS6 | RegA/Rls-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCAGCGAGAGCGAGGCGCCGGCGTTGTG |
| Internal bar code: | TTTGGTTAAGATGATAGTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 60 |
| LEAP-Seq percent confirming: | 84.2991 |
| LEAP-Seq n confirming: | 451 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAACAACTAAACGGGCG |
| Suggested primer 2: | AGGTCTGGATGCCTGTATGG |