| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093193 |
| Chromosome: | chromosome_15 |
| Location: | 1198847 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre15.g641266 | TPT22 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACCCTCTCCCGCCTCCCCCCATCAACACC |
| Internal bar code: | CATGTACGTGTTAGCACGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 96.8504 |
| LEAP-Seq n confirming: | 123 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGTTGTGGTCGGGAGTTG |
| Suggested primer 2: | GAGGTATGGGCGTATGTGGT |