| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093194 |
| Chromosome: | chromosome 13 |
| Location: | 455711 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564850 | (1 of 1) PTHR31651:SF3 - AUXIN EFFLUX CARRIER FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCCCTTCCCTCCCGCGTCTTTCATTTG |
| Internal bar code: | CCTCTTCCACGGCGATCCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 20 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCTCTGCTGGTGATGCT |
| Suggested primer 2: | ATACTGGCATGTCGTAGCCC |