| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093202 |
| Chromosome: | chromosome 5 |
| Location: | 2877424 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCTTCGTCTCGGTGTACGTGATTCTT |
| Internal bar code: | CTTACATATAGCCCCCGGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 96.2185 |
| LEAP-Seq n confirming: | 458 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAGCAACGACGCTACAC |
| Suggested primer 2: | GGGAACATCACGTAGCCAGT |