Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.093213 |
Chromosome: | chromosome_14 |
Location: | 1582182 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g618750 | FAP246 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGTTTGGCAGGGTCCGGCCCGAAGCGCACC |
Internal bar code: | GACGCGTCCGGCGCGTATGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 284 |
LEAP-Seq percent confirming: | 91.3632 |
LEAP-Seq n confirming: | 4115 |
LEAP-Seq n nonconfirming: | 389 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGGAGGTGTTGGATGTGTG |
Suggested primer 2: | GCATGCTAACAGCCTTCTCC |