Insertion junction: LMJ.RY0402.093214_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.093214
Chromosome:chromosome 6
Location:3468433
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g278095 (1 of 1) IPR016024//IPR027417 - Armadillo-type fold // P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCAGCGCGCCGCTAGTATTCTGGTGGCA
Internal bar code:GAATATACCCACGTGGTCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:79
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCCCGCACAAGAATGTC
Suggested primer 2:GAGGAGGACGAGGAGGAGAT