Insertion junction: LMJ.RY0402.093221_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.093221
Chromosome:chromosome 17
Location:6950983
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g746047 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCCTCCCCCCGTCCCCCAACCCATCCA
Internal bar code:TACGTCCGGGCTTGGCCCAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:323
LEAP-Seq percent confirming:98.708
LEAP-Seq n confirming:1910
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGCAAATAGTAACGTGGT
Suggested primer 2:CCCAGAGTGGAGAGGACAAA