| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093257 |
| Chromosome: | chromosome 13 |
| Location: | 2531602 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580550 | HLM20 | (1 of 2) K11426 - SET and MYND domain-containing protein (SMYD); putative N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAAGCCTTTGCGCGCGGGTGCGGTGTGT |
| Internal bar code: | GCGCGACTCTTAGAGACTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 99.727 |
| LEAP-Seq n confirming: | 1461 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGATTAGATCTGCGAGC |
| Suggested primer 2: | GCTACGAGGCAAAACTCCTG |