| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093262 |
| Chromosome: | chromosome 14 |
| Location: | 1683985 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619300 | CYN16 | Cyclophilin 16; (1 of 60) 5.2.1.8 - Peptidylprolyl isomerase / Rotamase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGGACGTTTGAAATCCCGTATCGTGATG |
| Internal bar code: | TTTAACGACCCCGGTATATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 434 |
| LEAP-Seq percent confirming: | 97.291 |
| LEAP-Seq n confirming: | 11780 |
| LEAP-Seq n nonconfirming: | 328 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGTGGACACTAATCCCA |
| Suggested primer 2: | CAACTTCAAATGCCTGCTCA |