| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093280 |
| Chromosome: | chromosome 2 |
| Location: | 5177764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105550 | (1 of 1) IPR000104//IPR003008 - Antifreeze protein, type I // Tubulin/FtsZ, GTPase domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAATGGCGGTATTATGCATCCCCTCAGC |
| Internal bar code: | GTGCACTAGGTGCGGAGCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 98.0796 |
| LEAP-Seq n confirming: | 2094 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTCAAAGTTGGAACCCT |
| Suggested primer 2: | ACATTAAAGTGCAGTGCCCC |