| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093284 |
| Chromosome: | chromosome 14 |
| Location: | 1357862 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617150 | RLS4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAAGTAAGCAGTGGAGCTCACATTCGT |
| Internal bar code: | CGTACCTACTCTTGTTGGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 98.9546 |
| LEAP-Seq n confirming: | 4733 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATGCTTGCATTGGTTGG |
| Suggested primer 2: | CCTCCGTTTTGACTGGTGTT |