| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093284 |
| Chromosome: | chromosome 14 |
| Location: | 1357865 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617150 | RLS4 | 5'UTR/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAGCGGAAAACTGGGACGAGCCCGCTG |
| Internal bar code: | GGCATATATTCGCCGAGGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1128 |
| LEAP-Seq percent confirming: | 99.414 |
| LEAP-Seq n confirming: | 14928 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATGCTTGCATTGGTTGG |
| Suggested primer 2: | CCTCCGTTTTGACTGGTGTT |