| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093301 |
| Chromosome: | chromosome 6 |
| Location: | 7610421 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301201 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTAACATCCGCATCCATACATGCGCTGA |
| Internal bar code: | ACGGGCGCTTCTGAATACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 418 |
| LEAP-Seq percent confirming: | 80.3153 |
| LEAP-Seq n confirming: | 1885 |
| LEAP-Seq n nonconfirming: | 462 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTTGGGACTGCACTGTT |
| Suggested primer 2: | TTCTGGAATTTGGATGAGCC |