Insertion junction: LMJ.RY0402.093305_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.093305
Chromosome:chromosome 6
Location:1940296
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g263650 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCCACCATGCCGATCATGGCGCGCACG
Internal bar code:TTCTCAGCTTTTGTACACTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:271
LEAP-Seq percent confirming:72.0198
LEAP-Seq n confirming:4646
LEAP-Seq n nonconfirming:1805
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGTCCTTCCTCTTTCGT
Suggested primer 2:CCTCCCCTGGGTCTTAATGT