| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093314 |
| Chromosome: | chromosome 3 |
| Location: | 7290908 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207000 | (1 of 1) PTHR34543:SF1 - ABSCISIC ACID (ABA)-DEFICIENT 4 PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCAATTGAGCGCCTCCTGCAACCCCTGT |
| Internal bar code: | TAAAGTCGATCTTTTGTCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.6059 |
| LEAP-Seq n confirming: | 1011 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCGTCCAACATAAGA |
| Suggested primer 2: | ATTGTGCTGGTGAGTTTCCC |