| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093325 |
| Chromosome: | chromosome 12 |
| Location: | 4508722 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTTGACGTAGTGTGCATGCGCAGGGGA |
| Internal bar code: | AGTGGGTAATTAAGGGGAACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 88.1279 |
| LEAP-Seq n confirming: | 3474 |
| LEAP-Seq n nonconfirming: | 468 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTACTCCCAGTCCAGGT |
| Suggested primer 2: | CTGCCTCCAATCCTCCATTA |