| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093327 |
| Chromosome: | chromosome_10 |
| Location: | 220451 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g419200 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGCCTATGGGATGGGCCTCGCCTCCACCC |
| Internal bar code: | ACGGACTCCCTGGCGTGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 96.5399 |
| LEAP-Seq n confirming: | 12081 |
| LEAP-Seq n nonconfirming: | 433 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGGCACTCCATTACCAG |
| Suggested primer 2: | AGCAACGTGCAATGTGAGAG |