| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093332 |
| Chromosome: | chromosome 7 |
| Location: | 5177519 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348450 | (1 of 18) K13412 - calcium-dependent protein kinase (CPK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCATGCCGCAGAACCGCACACCCAAAT |
| Internal bar code: | GATGCTCAGAAATCTCATCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 85.7887 |
| LEAP-Seq n confirming: | 2469 |
| LEAP-Seq n nonconfirming: | 409 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATACACTTGCATTGTGC |
| Suggested primer 2: | ATGCATGCCAAACGTACAAA |