Insertion junction: LMJ.RY0402.093339_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.093339
Chromosome:chromosome 11
Location:1016333
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467666 LCI13,GT90F43,GT90-43 Low-CO2-inducible 13; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCGCCTACCTGCCTTCCCAGAACGTCA
Internal bar code:AATCCTAGTATTGCTTGGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:615
LEAP-Seq percent confirming:71.8785
LEAP-Seq n confirming:1301
LEAP-Seq n nonconfirming:509
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:AGACGGCTAACCCATCCTCT
Suggested primer 2:CCCATAGTAACGAGGTGCGT