| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093346 |
| Chromosome: | chromosome 12 |
| Location: | 3956272 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516450 | CAG1 | Gamma carbonic anhydrase 1; (1 of 1) PTHR13061//PTHR13061:SF7 - DYNACTIN SUBUNIT P25 // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCACATGCAAACGCTGTCCGCGACCAGA |
| Internal bar code: | GTGAGCACTTTGTGGCTCACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 98.3759 |
| LEAP-Seq n confirming: | 4664 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGTTGAAGATGCTGTC |
| Suggested primer 2: | TAGCTAGCTTGTGGCCTGGT |