| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093347 |
| Chromosome: | chromosome 13 |
| Location: | 2137681 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578012 | (1 of 1) PTHR11061//PTHR11061:SF29 - RNA M5U METHYLTRANSFERASE FAMILY // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGCTGGTGCTGCCGTACGTGGCGGAGG |
| Internal bar code: | CCCGAGCTCCGCGTCGCACTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 88 |
| LEAP-Seq percent confirming: | 99.5575 |
| LEAP-Seq n confirming: | 1350 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGTTGAGCCAGATGGAG |
| Suggested primer 2: | CGCGTCACTAAAGCACTGAA |