| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093361 |
| Chromosome: | chromosome 5 |
| Location: | 2725306 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236802 | (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTAAGGGTGGCTGGCACCAAGTTAAAGG |
| Internal bar code: | GGCATCGCTGACTATGTCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 124 |
| LEAP-Seq percent confirming: | 73.6842 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCACCATGAACCCAGTCA |
| Suggested primer 2: | AGCGGCATACTTTGTTTGCT |