Insertion junction: LMJ.RY0402.093371_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.093371
Chromosome:chromosome 16
Location:1020834
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g649150 (1 of 1) PF13414//PF13920 - TPR repeat (TPR_11) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGTGCATGGACGCCGAGCGAGGTGAGC
Internal bar code:GCCGTGGACCGAAGGTTCGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:192
LEAP-Seq percent confirming:98.917
LEAP-Seq n confirming:548
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCAATCCCATGAAACACC
Suggested primer 2:ATACACACCGTTCGCATGAA