| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093384 |
| Chromosome: | chromosome 1 |
| Location: | 5796456 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGGGAAGCGTGGCCCGCTAAACTAAA |
| Internal bar code: | AGTTACGTACCCGGTTTGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 81.6949 |
| LEAP-Seq n confirming: | 13130 |
| LEAP-Seq n nonconfirming: | 2942 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCCATTAACAACGGCAGC |
| Suggested primer 2: | CTTTCGCTAACTCCGTCCTG |