| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093426 |
| Chromosome: | chromosome_10 |
| Location: | 82297 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g418150 | FAP178 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAGCACTTGCAACGACGCACCTGATGCG |
| Internal bar code: | TTATGTGGGGCTTATTTGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 92.1208 |
| LEAP-Seq n confirming: | 1403 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGCTTCGCAATCACAAA |
| Suggested primer 2: | CACGAGCGTGACTTGTTGTT |