| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093431 |
| Chromosome: | chromosome 7 |
| Location: | 1803970 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325725 | (1 of 1) 1.1.1.47 - Glucose 1-dehydrogenase (NAD(P)(+)) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTATATGCTCTTAAGGGAAATTGGTGTAC |
| Internal bar code: | GAATGTACGCACGGACCAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 99.8596 |
| LEAP-Seq n confirming: | 1423 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGAAATGGAGATGCAG |
| Suggested primer 2: | ATCCAGCACAATGCACCATA |