Insertion junction: LMJ.RY0402.093440_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.093440
Chromosome:chromosome 17
Location:3935693
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g728450 (1 of 1) IPR006626//IPR010308//IPR011050 - Parallel beta-helix repeat // TRP-like family // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAAGAACGCGCCGCCCCCCTGCGCCGCC
Internal bar code:CGTCGAGCCGTCTTTGTGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:765
LEAP-Seq percent confirming:67.4528
LEAP-Seq n confirming:286
LEAP-Seq n nonconfirming:138
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTACCTGCCTTCCTGCTGTC
Suggested primer 2:GCTACTGTTGCCTGACACGA