| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093456 |
| Chromosome: | chromosome 8 |
| Location: | 4941634 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385400 | (1 of 3) 3.5.1.1 - Asparaginase / L-asparagine amidohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTCGGAGGCCGGGAAAGGCGAGCACGA |
| Internal bar code: | AGTCCGTTCGATTACCAGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1148 |
| LEAP-Seq percent confirming: | 99.2655 |
| LEAP-Seq n confirming: | 946 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCGGAGTCACCTCTACT |
| Suggested primer 2: | GGCAGTGGCTCCAAGTACAG |