Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.093461 |
Chromosome: | chromosome_6 |
Location: | 5172562 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
None |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACAGACTTCACGACGAGTTCAGCATGATAG |
Internal bar code: | GGTTGGAAACTCGGTCACCCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 25 |
LEAP-Seq percent confirming: | 93.1694 |
LEAP-Seq n confirming: | 341 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGTCTAGCTCATCGACGGA |
Suggested primer 2: | CTGTCGTTGCTGACGTTGAT |