Insertion junction: LMJ.RY0402.093466_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.093466
Chromosome:chromosome 11
Location:39716
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467526 (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGACTGAGAGGGCCGCTCTTTTGCCGCGG
Internal bar code:TCTCCACCTCCTCCGTTTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:347
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:1705
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCACCCCACTTGGAACT
Suggested primer 2:CAACCACATGCTGGAATCAC