| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093477 |
| Chromosome: | chromosome 6 |
| Location: | 7790272 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302426 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATACTGAGCCGCGCTCAGCCATCTTATC |
| Internal bar code: | TTCGACACATGGGGTCGGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 98.7944 |
| LEAP-Seq n confirming: | 7375 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGACTGATGAGCGATGAG |
| Suggested primer 2: | GTATTCCTGACACGGGCTGT |