| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093536 |
| Chromosome: | chromosome 3 |
| Location: | 7208341 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207901 | (1 of 6) 3.6.4.1 - Myosin ATPase / Actomyosin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGGCCGGCTGTGGGGCGGCTTAAGGAA |
| Internal bar code: | GGGTAATATGGCTCACTACACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 83.5228 |
| LEAP-Seq n confirming: | 14330 |
| LEAP-Seq n nonconfirming: | 2827 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGACAGCGCATAGTAGACG |
| Suggested primer 2: | AGAGGCTACAGCCCAGTCAA |