| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093560 |
| Chromosome: | chromosome 3 |
| Location: | 711688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146047 | (1 of 6) 3.1.1.1 - Carboxylesterase / Procaine esterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCCTACTACACACACCGCACATCTAGC |
| Internal bar code: | GAAATAAGTCCCAATCGTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.0123 |
| LEAP-Seq n confirming: | 5614 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTAAGCGAGTCCACGAG |
| Suggested primer 2: | GAAGTGCATGTCACTGTGGG |