| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093580 |
| Chromosome: | chromosome 14 |
| Location: | 1280387 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616600 | FZL,FZL1 | Plastid-localized; (1 of 1) PTHR11649//PTHR11649:SF36 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // FZL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGGCTTGTATGTAGTGATAGGAGGAGG |
| Internal bar code: | GTCTAGGCAGTGGGCAAGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 77.7274 |
| LEAP-Seq n confirming: | 2401 |
| LEAP-Seq n nonconfirming: | 688 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAAAGGCGAACGGTGTAA |
| Suggested primer 2: | CGGCCCTACACATGAAGAAT |