| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093601 |
| Chromosome: | chromosome 3 |
| Location: | 7437313 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205850 | MAE6,MFT6 | MATE efflux family protein; (1 of 3) PTHR11206//PTHR11206:SF95 - MULTIDRUG RESISTANCE PROTEIN // MULTI ANTIMICROBIAL EXTRUSION FAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGACGCGGTCACGGCCAAGGTGCGGGAC |
| Internal bar code: | GGTTAGTCGCTAGCTCGAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 99.8655 |
| LEAP-Seq n confirming: | 10394 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCAGGACCAGACCGTAG |
| Suggested primer 2: | GAGTTTAACCTCGGGCATGA |