Insertion junction: LMJ.RY0402.093612_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.093612
Chromosome:chromosome 17
Location:6882305
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g745647 (1 of 1) PF09350 - Domain of unknown function (DUF1992) (DUF1992) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCAGACTCCACACGGGGGACACGCCCGG
Internal bar code:CCGTTGTTGGTCATCAGCTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:252
LEAP-Seq percent confirming:99.631
LEAP-Seq n confirming:1080
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAACAGACTGACCCCATGCT
Suggested primer 2:CCATGTTACTGAGGCCAGGT