Insertion junction: LMJ.RY0402.093644_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.093644
Chromosome:chromosome 1
Location:4027012
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g026150 (1 of 3) 3.4.24.59 - Mitochondrial intermediate peptidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACCATCTTCGTCATCTTCCTGCAGGTGC
Internal bar code:GTGGATCGTGCCTGACAAGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:63.9857
LEAP-Seq n confirming:2864
LEAP-Seq n nonconfirming:1612
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCACTTGCTTGTAGGGAA
Suggested primer 2:TTAAGCATAGGGTTGGGTGG