Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.093651 |
Chromosome: | chromosome 17 |
Location: | 181503 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGCGACTTAAATCGGGCAGACCAGACT |
Internal bar code: | GGAGTCTAGGCCCACAGCATGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 270 |
LEAP-Seq percent confirming: | 30.5216 |
LEAP-Seq n confirming: | 749 |
LEAP-Seq n nonconfirming: | 1705 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGAGCTCAAGCAGGGTGTAG |
Suggested primer 2: | CAGCCGTCGTACAATCACAC |