| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093662 |
| Chromosome: | chromosome 7 |
| Location: | 1819621 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325728 | (1 of 5) PF12681 - Glyoxalase-like domain (Glyoxalase_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAAAGAGGTGGGTGGCGGGCTAGATGGC |
| Internal bar code: | GGTCGCCCTGATCTGTTAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 99.0446 |
| LEAP-Seq n confirming: | 311 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCGAAGGCTCCGTAGTA |
| Suggested primer 2: | TATTCGCAGCAACTGAATGC |