| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093672 |
| Chromosome: | chromosome_13 |
| Location: | 3742365 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g589450 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACAGACGCACGCGCCACACCTCCTCCGCA |
| Internal bar code: | TGGGTTACTGGGGGCTCTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 78.0854 |
| LEAP-Seq n confirming: | 677 |
| LEAP-Seq n nonconfirming: | 190 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGAAAAGAATGGTGATG |
| Suggested primer 2: | CGATACCAAGGAGACCCTGA |