| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093676 |
| Chromosome: | chromosome 14 |
| Location: | 535308 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611517 | (1 of 3) K07393 - putative glutathione S-transferase (ECM4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGAGGACAGACAAGTTGGGTGATGGGG |
| Internal bar code: | ATGCACGTAGTGTATGATAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.8826 |
| LEAP-Seq n confirming: | 5955 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACAGCGCGATCAACAAC |
| Suggested primer 2: | TTTCGTTTAGTTTGGGCTGG |