| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093679 |
| Chromosome: | chromosome 8 |
| Location: | 870667 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361250 | (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGCGCCGTGTCAGCACCGCCTTCTTCC |
| Internal bar code: | TGTTGGGGCGGAAACCCTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 71 |
| LEAP-Seq percent confirming: | 98.7288 |
| LEAP-Seq n confirming: | 1398 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAAGTGGGTCTGGTCGT |
| Suggested primer 2: | ACGAGGAGGAGGAGGAAGAG |