Insertion junction: LMJ.RY0402.093683_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.093683
Chromosome:chromosome 16
Location:1080659
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g649550 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGATGAGGGATTCGTGTCTGGTGTGTGGT
Internal bar code:TCGCAACTCACCGGGAGACTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:334
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:507
LEAP-Seq n nonconfirming:169
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACACATACCGTTTTGCTGG
Suggested primer 2:GGAGCTTGCCCTTGTAACTG