| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093724 |
| Chromosome: | chromosome 1 |
| Location: | 624713 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003500 | CLUB1,MSH2 | (1 of 1) IPR017987 - Wilm's tumour protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGATGTTGGGCACGGCGGTGGCGGCGGC |
| Internal bar code: | CTCCAGGTACGCCCCGGCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 87.3786 |
| LEAP-Seq n confirming: | 360 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAAAAGCGCTGTAACCCC |
| Suggested primer 2: | TTTGCAACCTAGTGCCCTCT |