| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093744 |
| Chromosome: | chromosome 2 |
| Location: | 8549779 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141946 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCCACAGCTCCTACCTTTTCATCAGCA |
| Internal bar code: | GTGTTTCGGGTGCCAAAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 73.9023 |
| LEAP-Seq n confirming: | 5083 |
| LEAP-Seq n nonconfirming: | 1795 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGTCACTCCCCACAGT |
| Suggested primer 2: | TGCCCCTTCAATTGTAATCC |