| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093757 |
| Chromosome: | chromosome 6 |
| Location: | 4059044 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278211 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCGTCGAGCGCCTGCGCCGTGATGAT |
| Internal bar code: | GAAGGCAGGGTGAGGCGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 98.8136 |
| LEAP-Seq n confirming: | 583 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCCATTCAATCCCAAGC |
| Suggested primer 2: | ATAAGCACAATACCTGCCCG |