Insertion junction: LMJ.RY0402.093782_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.093782
Chromosome:chromosome 6
Location:3529170
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278110 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACGCATGGTCCAAAGAACGCATGATGGG
Internal bar code:CACTAGTCGAGTCGTTTATAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:516
LEAP-Seq percent confirming:98.0378
LEAP-Seq n confirming:3997
LEAP-Seq n nonconfirming:80
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GATCCCAGCTACCACACCAC
Suggested primer 2:GCCGTCTCACTGTCTTAGGC