Insertion junction: LMJ.RY0402.093790_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.093790
Chromosome:chromosome 13
Location:4088569
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g591400 COA1,COAA1 (1 of 1) 2.7.1.33 - Pantothenate kinase / Pantothenic acid kinase; Pantothenate kinase, CoA biosynthesis CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCCGCTGACGTCACCGCACCCGTACGG
Internal bar code:GCGTTTGCCCGATGGTCCCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:474
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGGCAACAGGTGATGTG
Suggested primer 2:AGAGGCAGTAGCAGAGGCAG