Insertion junction: LMJ.RY0402.093825_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.093825
Chromosome:chromosome 17
Location:1740264
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g709000 (1 of 1) PTHR11319//PTHR11319:SF35 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCAGCTGCACCAACTGTACAATTCAGAA
Internal bar code:CCCCAGTCTCTCGGCCGACGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:55
LEAP-Seq percent confirming:54.3243
LEAP-Seq n confirming:201
LEAP-Seq n nonconfirming:169
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGACGAACCCTACTCCAA
Suggested primer 2:CACCTGGATGTTGTCTGGTG