| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093831 |
| Chromosome: | chromosome 12 |
| Location: | 6465161 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538200 | (1 of 1) PTHR23305//PTHR23305:SF1 - GTP-BINDING PROTEIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGGCATCCCCGAACACGCACGCGCGCA |
| Internal bar code: | GGTTGCAGGTAAGCGTTTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 47.9263 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCTTCCTTTCATTTCCC |
| Suggested primer 2: | ATGGTGGTGCATATGGGTTT |